Wednesday, February 22, 2012
Evidence for an XMRV-like sequence in prostate cancer?
Just saw this one today, published in the British Journal of Cancer, a respectable journal-- despite the title of the paper, "No evidence for the involvement of XMRV or MCV in the pathogenesis of breast cancer.", the paper goes on to describe single-round PCR amplification of an XMRV type sequence in two cases of prostate cancer (out of 12). They had optimized the reaction so as to detect 700 copies of purified plasmid DNA -- one sample did not elicit sufficient product to clone, but the other did, and upon sequencing it was the same as the VP62 sequence with two exceptions - indicating it is not likely to be contamination from the plasmid the investigators used as a control. One caveat, the group does not appear to have tested the tissues for mouse DNA -- and they acknowledge this by admitting that the sequence could be from exogenous DNA. If you have access to the journal, you can read the paper here.